JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327582.1
Database:	nt	Go to original record
Description:	Fagus sylvatica tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Fagus sylvatica
Original size:	611 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatggaa
51    acttaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctattttccg aaaacaaata agggttgaga
151   agaaagcaag aataaaataa aaaaaaaaaa ggataggtgc agagactcaa
201   tggaagatgt tctaacaaat ggggttgact tcgttacgtt attaaattca
251   agtaatcctt ctatcaaaac tacagaaagc atgaaggata aacctatata
301   catacgtata tgtactgaaa tcctatctca aatgattaat gacgaccgga
351   atctttattt atttatattt ctataaataa ataccgaaag agttgttgtg
401   aatcgatcca aattgaagaa agaatcgaat atttattaat tcaattattt
451   actccatcat agtctgatag atcttttgaa gaactaattt atcgtacgag
501   aataaagata gagtcccatt ctacatgtca ataccgacaa gaatgaaatt
551   tatagtaaga ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details