JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327576.1
Database:	nt	Go to original record
Description:	Acer pseudoplatanus tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Acer pseudoplatanus
Original size:	596 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaataaaac aaagcaaaca
151   agggttcaga aagcgagaaa agggataggt gcagagactc aacggaagct
201   gttctaacaa atggagttga ctgccttttt ttgggaaaga aaggaaaatt
251   aatcgaatat cgaaactgca taaaggatga aggataagtc tataatacac
301   tatggataca aaatgaaaaa ttatctcaaa actgacaacc tgaagctgta
351   tttatattaa tgaaaaagaa aacaaaagaa ttgggattcc aagttgaaga
401   atcaaatatt cattgatcaa attattcact ccatagtctg gtagatcgtt
451   tcttttgaag aactgattac tcagacgaga ataaagatag agtcccattc
501   tacatgtcaa tataaatacc ggcaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details