JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327573.1
Database:	nt	Go to original record
Description:	Alnus glutinosa tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Alnus glutinosa
Original size:	609 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaata aaacaaattg
151   aagggttcat aaagcgagac taaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaattggg gttgacttcg ttccgttatt aaagtaatcc
251   ttctatcaaa actacagaaa ggatgaagga taaccgtata tacatacgta
301   tacgtactga aatactatct caaaaaatta atgacgacct gaatctttat
351   ttattttttt ttatatttat ataaaaactg aatgaaagag ttgttgtgaa
401   tcgatccaaa ttgaagaaag aatcgaatat ttattaatca aattatttac
451   tccatcagag tctgatagat cttttgaaga actaattaat cggacgagaa
501   taaagataga gtcccattct acatgtcaat accgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details