JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327572.1
Database:	nt	Go to original record
Description:	Acer campestre tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Acer campestre
Original size:	594 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaataaaac aaagcaaaca
151   agggttcaga aagcgagaaa ggggataggt gcagagactc aacggaagct
201   gttctaacaa atggagttga ctgccttttt ttgggaaaga aagggaaatt
251   aatcgaatat cgaaactgca taaaggataa gtttctaata cactatggat
301   tggatacaaa atgaaaaatt atctcaaaaa tgacaacctg aagctgtatt
351   tctattaatg aaaaagaaaa caaaagaatt gggattccaa gttgaagaat
401   cgaatattca ttgatcaaat cattcactcc atagtctggt agatcgtttc
451   ttttgaagaa cttattactc agacgagaat aaagatagag tcccattcta
501   catgtcaata taaataccgg caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details