JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327571.1
Database:	nt	Go to original record
Description:	Abies alba tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Abies alba
Original size:	558 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	558	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagaaaaggg tttctctcct
151   tctcctaagg aaagggatag gtgcagagac tcaatggaag ctattctaac
201   gaatgaatct catttggggt ccaatactct atttatagaa cgttctattt
251   acacctcgaa agtaggaatg ttatataaca tcaaacaaaa ctcgcgatca
301   gaacttgaat tgttccaagc attttattcg taaaatagat gccagattcg
351   agttgaagta atgattttac attaagtaat ccaattatga acttatctac
401   tctagatagg gagttgaatc agtttttgga ataaatgatt ggacgaggat
451   aaagatagag tccaattcta cgtgtcaatg taaacaacaa tgcaaattgc
501   agtagaagga aaatccgttg gctttataga ccgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details