JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ847406.1
Database:	nt	Go to original record
Description:	Pentas parvifolia chloroplast DNA for trnT-L-F region
Taxon:	Rhodopentas parvifolia
Original size:	1697 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	725	1306	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcggtagtc gagaagcctt ttcatcaaaa attcataaag
251   tctaagtagt aaagatgaag tgaaggataa acgtatatac gtagtaaata
301   gtatattaaa tgagtaatga cagcccgaat gaatctatat ttttttctat
351   gaacaaataa agaattgttg ttagtagatt ccgcgtttaa gaaagaatca
401   aatattcatt gatcaaatga ttcactccat agtctgatcg atcttttcac
451   caactgatta atcggacgag aataaagata gagtcccatt ctacatgtca
501   atgccggcaa caatgaaatt tatagttaag aggaaaatcc gtcgactttt
551   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details