JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ847400.1
Database:	nt	Go to original record
Description:	Ixora narcissodora chloroplast DNA for trnT-L-F region
Taxon:	Ixora narcissodora
Original size:	1696 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	769	1332	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aggggcaatc ctgagccaaa tcctgttttc cgaaaccaaa ggtacagaaa
151   gtgaaaaggg gataggtgca gagactcaac ggaagttgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaacctttc catcgaaaat tccgaaagga
251   taaagtgaaa gataaaggta tatacgtatt gaatactata tcaaatgatt
301   aatgacgact cgaatgaatc tgtatttttt atatgaaaag ggaagaattg
351   gtgtgaatag attccacatt aaagaaagaa tcgaatattc attgattaaa
401   tgattcactc catagtctga tagatctttt caagaattga ttaatcggac
451   gagaataaag atagagcccc gttctacatg tcaatgtcgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details