JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ854519.1
Database:	nt	Go to original record
Description:	Phleum pratense chloroplast partial tRNA-Leu (UAA) gene, intron, specimen voucher Gr 3801/82 Brudzynska (IPK)
Taxon:	Phleum pratense
Original size:	613 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	613	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt gttagtggga ttccttctaa
251   attcgagaaa gaagggcttt atacatctaa taaacacgta tagatactga
301   catagcaaac gattaatcac agaacccata ttataatata ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaataaaaa attctgaatt
401   tttttagaat tattgtgaat ccattccaat cgaatattta gtaatcaaat
451   ccttcaattc aaagttttga ggtctttaaa aaagtggatt aatcggacga
501   ggacaaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttctagtaaa aggaaaatcc gtcgacttta taagtcgnga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details