JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ620159.1
Database:	nt	Go to original record
Description:	Psydrax locuples chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Bremer et al. 4290 (UPS)
Taxon:	Psydrax locuples
Original size:	1659 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	785	1326	+	542 bp

Amplicon:

542 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agttaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tgacgactcg
301   actgaatctg tattttttat atgaaaatgg aagaatagat tccacattga
351   agaaagaatc gaatattcat tgattaaatg attcactcca tagtctgata
401   gatcttttca agaattgatt aatcggacga gaataaagat agagtcccgt
451   tctacatgtc aatgtcggca acaatgaaat ttatagtaag aggaaaatcc
501   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	542	523	2.44e-08

Match details