JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ620152.1
Database:	nt	Go to original record
Description:	Pachystigma pygmaeum chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Pawek 12335 (UPS)
Taxon:	Vangueria pygmaea
Original size:	1731 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	808	1363	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcga tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacaaa cgtaaacgta ttcaatacta tatcaaatga ttaatgacga
301   ctcgactgaa tccgtatttt ttatatgaaa acggaagagt tggtgtgaat
351   agattccaca ttgaagaaag aatcgaatat tcattgatta aatgattcac
401   tccatagtct gatagatctt ttcaagaatt gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details