JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ620141.1
Database:	nt	Go to original record
Description:	Fadogiella stigmatoloba chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Lawton 1318 (S)
Taxon:	Fadogiella stigmatoloba
Original size:	1667 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	757	1309	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaac cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactataat caaatgatta atgacgcctc
301   gactgaatcc gtatttttta tatgaaaacg gaagaattgg tgtgaataga
351   ttccacattg aagaaagaat cgaatattca ttgattaaat gattcactcc
401   atagtctgat agatcttttc aagaattgat taatcggacg agaataaaga
451   tagagtcccg ttctacatgt caatgtcggc aacaatgaaa tttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details