JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ586101.1
Database:	nt	Go to original record
Description:	Tetraselago wilmsii chloroplast trnT-trnL intergenic spacer, trnL gene, and trnL-trnF intergenic spacer, specimen voucher Bremer 4336 (UPS)
Taxon:	Tetraselago wilmsii
Original size:	1762 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	842	1402	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ttcaaaacaa aggttcagaa
151   ggcaaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttgact tcgttggtag aggaatctct ccatcgaaac ttcagaaagg
251   gtgacgggta aacgtatcta ttgaataccc tatcaaaatc aaatgattaa
301   tggtgccccg aatccgtatc tgtatttttg atataaaaag tggaaaattt
351   ggtgtgaatt tattccacca ttgaagaaag aattaactaa aatattcatt
401   catcaaatca ttcactccat agtctgataa gatctgatta attggacaag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details