JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ586071.1
Database:	nt	Go to original record
Description:	Selago impedita chloroplast trnT-trnL intergenic spacer, trnL gene, and trnL-trnF intergenic spacer, specimen voucher Bremer 4381 (UPS)
Taxon:	Selago impedita
Original size:	1732 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	804	1373	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aataataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaacaaa ggttcagaag
151   gcaaaaaagg gataggtgca gagactcaat ggaagttgtt ctaacaaatg
201   gagttgactt cgttggtaga ggaatctctc catcgaaact tcagaaaggg
251   tgacgggtaa acgtatctat tgaatactct atcaaaatca aatgattaat
301   ggtgccccga atccgtatct gtatttttga tctaaaaggt ggaaaatttt
351   gtgtgaattt attccaccat tgaagaaaga attaactaaa atattcattc
401   atcaaatcat tcactccata gtctgataga tcttttaaag atctgattaa
451   ttggacaaga ataaagatag agtcccattc tacatgtcaa taccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details