JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ586063.1
Database:	nt	Go to original record
Description:	Selago myriophylla chloroplast trnT-trnL intergenic spacer, trnL gene, and trnL-trnF intergenic spacer, specimen voucher Bremer 4393 (UPS)
Taxon:	Selago myriophylla
Original size:	1747 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	820	1387	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agttcagaag
151   caaaaaaggg atagtgcaga gactcaatgg aagttgttct aacaaatgga
201   gttgacttcg ttggtagagg aatctctcca tcgaaacttc agaaagggtg
251   acgggtaaac gtatctattg aatactctat caaaatcaaa tgattaatgg
301   tgccccgaat ccgtatctgt atttttgatc taaaaagtgg aaaattttgt
351   gtgaatttat tccaccattg aagaaagaat taactaaaat attcattcat
401   caaattattc actccatagt ctgatagatc tttaaaagat ctgattaatt
451   ggacaagaat aaagatagag tcccattcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details