JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ551271.1
Database:	nt	Go to original record
Description:	Buddleja polystachya chloroplast tRNA-Leu (UAA) gene
Taxon:	Buddleja polystachya
Original size:	1363 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	400	964	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag gtataacttt caaattcaga gaaaccccgg aattnnnnaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttaact gcgttggtag cggaatcttt cgatcgaaac ttcagaaaga
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   accgaatctg tatctgtatt tttttatatg aaaaatggaa aaattggtgt
351   gaatttattc cacattgaag aaagaatcnn nnnnnatatt cattcatcaa
401   atcattcact ccatagtctg atagatcttt taaagatctg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details