JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ550527.1
Database:	nt	Go to original record
Description:	Lyperia tristis chloroplast tRNA-Leu (UAA) gene, specimen voucher Vlok J.H.L. 2488 (S)
Taxon:	Lyperia tristis
Original size:	1656 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	696	1253	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcaaaataa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatcca tttcatcaaa cttcagaaag
251   ggtaatggat aaacgtatct attgaatact atatcaaatg attaaggccc
301   gaactgtgta gttttttata taaaaaatgg aaaaatgggt gtgaatttat
351   ttcaccgttg aagaaagaat caaataaaat attcattcat caaatcattc
401   actccatagt ctgatagatc ttttaaggaa ctgattaatc ggacgagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg atttaaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details