JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ550525.1
Database:	nt	Go to original record
Description:	Limosella grandiflora chloroplast tRNA-Leu (UAA) gene
Taxon:	Limosella grandiflora
Original size:	1598 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	626	1191	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcataa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgaat gcgttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   gtgatgaata aacgtatata ttgaatacta taataaaatt attaatttat
301   taatgatggc ccgatttctt tttttatatg aaaaatggaa aaatttgatg
351   tgaatttatt ccacattgaa gaaagaatca aatcaaatat tcatttataa
401   aataattcac tccaaagcct gatacatctt ttaaagaact gattaatcag
451   acgagaataa agatagagtc ccgttctaca tgtcaatacc ggcaaaaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details