JRC GMO-Amplicons | EURL GMFF

Record ID:	AY308502.1
Database:	nt	Go to original record
Description:	Hoffmannseggia erecta x Hoffmannseggia trifoliata isolate H5 chloroplast trnL gene, partial sequence; trnL-trnF intergenic spacer; and trnF gene, partial sequence.
Taxon:	Hoffmannseggia erecta x Hoffmannseggia trifoliata
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgtttttcg aaaaccaaga agagttcaga
151   aagggaaagg gagaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tacgtactga
301   aatactattt caattaatta caccagacag accccaaatc cctatttttt
351   aatatttata taacaaatga aagatgtgaa tagattccaa attgaagaaa
401   gaatctaata tttattgatc aaatcattca ctccatcata gtctgataga
451   tcttttgaag aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details