JRC GMO-Amplicons | EURL GMFF

Record ID:	AY308500.1
Database:	nt	Go to original record
Description:	Hoffmannseggia watsonii isolate 174 chloroplast trnL gene, partial sequence; trnL-trnF intergenic spacer; and trnF gene, partial sequence.
Taxon:	Hoffmannseggia watsonii
Original size:	1047 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctatcaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagcacaaat cctgtttttc gaaaaccaag aagagttcag
151   aaagggaaag ggagaaaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttgacgac atttcgtttc gttagtaaag gaatccttcc
251   atcgaaactc cagaaaagaa aggattaagg atgaacatat atacgtactg
301   aaatactatt tcaattgatt acaccagaca gaccccaaat ccctattttt
351   taatatttat atgacaaatg aaagatgtga atagattcca agttgaagaa
401   agaatctaat atttattgat caaatcattc actccatcat agtctgatag
451   atcttttgaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtca ataccgacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details