JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430756.1
Database:	nt	Go to original record
Description:	Hoffmannseggia ternata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Hoffmannseggia ternata
Original size:	1031 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg gagaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata taatatatac
301   gtactgaaat actatttcaa ttgattagac cagacagacc ccaaatccct
351   attttttaat atttatatga caaatgaaag atgtgaatag attccaagtt
401   gaagaaagaa tcgaatattt attgatcaaa tcattcactc catcatagtc
451   tgatagatct tttgaagaac tgattaatcg gacgagaata aagatagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttcta gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details