JRC GMO-Amplicons | EURL GMFF

Record ID:	AY308492.1
Database:	nt	Go to original record
Description:	Hoffmannseggia miranda isolate H21 chloroplast trnL gene, partial sequence; trnL-trnF intergenic spacer; and trnF gene, partial sequence.
Taxon:	Hoffmannseggia miranda
Original size:	1033 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	589	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaatttc aaattcagag aaaccctgga attaacaatg
101   ggcaatcctg agccaaatcc tgtttttcga aaaccaagaa gagttcagaa
151   agggaaaggg agaaaaggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttgacgacat ttcctttcgt tagtaaagga atccttccat
251   cgaaactcca gaaaagaaag gattaaggat gaacatatat acgtactgaa
301   atactatttc aattgattac accagacaga ccccaaatcc ctatttttta
351   atatttatat gacaaatgaa agatgtgaat agattccaag ttgaagaaag
401   aatcgaatat ttattgatca aatcattcac tccatcatag tctgatagat
451   cttttgaaga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaaagag aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details