JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430791.1
Database:	nt	Go to original record
Description:	Zuccagnia punctata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Zuccagnia punctata
Original size:	1060 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg aagaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaagctcc agaaaagaaa ggatcaagga tgaacatata tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccccaaa tccctatttt
351   ttaatattta tatgacaaat gaaagatgtg aatagattcc aagttgaaga
401   aagaaagaat cgaatattta ttgatcaaat cattcactcc atcatagtct
451   gatagatctt ttgaagaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details