Match details

Record ID: AF430790.1
Database: nt Go to original record
Description: Tachigali paniculata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Tachigali paniculata
Original size: 1063 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 575 + 575 bp

Amplicon: 575 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatacg tatacgtact
301   gaaatactat ttcaatttca attgattaga ccagacagac cccaaatctc
351   tattttttaa tatttatatg acaaatgaaa ggtgtgaatc gaatattcat
401   tgatcaaatc attcactcca tcatagtctg atagatcttt tgaagaactg
451   attaatcgga cgagaataaa gatagagtcc cattctacat gtcaataccg
501   acaacaatga aatttatagt aagaggaaaa tccgtcgact ttagaaatcg
551   tgagggttca agtccctcta tcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.59e-08
primerB 100.0 20 bp 0 0 575 556 2.59e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.