JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430787.1
Database:	nt	Go to original record
Description:	Stahlia monosperma chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Libidibia monosperma
Original size:	1072 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaaa aggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttgac gacatttcgt ttcgttagta aaggaatcct tccatcgaaa
251   ctccagaaaa gaaaggatca aggatgaaca tatatatacg tactgaaata
301   ttatttcaat tgattagacc agacagaccc caaatcccta ttttttaata
351   tttctatgac aaatgaaaga tttgaataga ttccaagttg aagaaagaat
401   cgaatattta ttgatcaaat cattcactcc atcatagtct gatagatctt
451   ttgaagaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details