JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430786.1
Database:	nt	Go to original record
Description:	Schizolobium amazonicum chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Schizolobium amazonicum
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	601	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   gggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaacga tgaacatacg tatacatact
301   gaaatactat ttcaattgat tagaccatta gaccagacgg accacaaatc
351   tctatttttg tcatatttat atgacaaatg aaagatgtga atcgattcca
401   agttgaagaa agaatcgaat attcattgat caaatcattc actccatcat
451   agtctgatag atcttttgaa gaactgatta atcggacgag aataaagata
501   gagtcccatt ctacatgtca ataccgacaa caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details