JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430784.1
Database:	nt	Go to original record
Description:	Parkinsonia aculeata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Parkinsonia aculeata
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaagc agagttcaga
151   aggggagaat aaaaaaaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttgacaac attttgtttc gttagtaaag gaatccttcc
251   atcgaaactc cagaaaagaa aggatcaagg atgaacatac gtatacgtac
301   tgaaatacta tttcaattga ttagaccaga cagaccccaa atctctattt
351   tttcatattt atatgacaaa tgaaagatgt gaatcgattc caagttgaag
401   aaagaatcga atattcattg atcaaatcat tcactccatc atagtctgat
451   agatcttttc aagaactgat tgagaataaa gatagagtcc cattctacat
501   gtcaataccg acaacaatga aatttatagt aagaggaaaa tccgtcgact
551   ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details