JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430783.1
Database:	nt	Go to original record
Description:	Pterolobium lacerans chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Pterolobium lacerans
Original size:	1088 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgttcct ttactaacga aacgaaatcc
251   ttccatcgaa actccagaaa agaaaggatc aaggatgaac atatatatac
301   gtatacgtac tgaaatacta tttcaattga ttagaccaga cagaccccaa
351   atctctattt ttgaatattt atatgacaaa tgaaagatgt gaatagattc
401   caagttgaag aaagaatcga atatttattg ataaaatcat tcactccatc
451   atagtctgat agatcttttg aagaactgat taatcggacg agaataaaga
501   tagagtccta ttctacatgt caataccgac aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details