JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430782.1
Database:	nt	Go to original record
Description:	Moullava spicata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Moullava spicata
Original size:	1103 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	605	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagtccaga
151   aagggaaagg gagaataaaa aaaaggatag gtgcagagac tcaacggaag
201   ctgttctaac aaatggagtt gacgacattt cgtttcgtta gtaaaggaat
251   ccttccatcg aaactccaga aaagaaagga tcaaggatga acatatatat
301   acgtatacgt actgaaatac tatttcaatt gattagacca gacagacccc
351   aaatctatat tttttaatat ttatatgaca aatgaaagat gtgaatagat
401   tccaagttga agaaagaatc gaatatttat tgatcaaatc attcactcca
451   tcatagtctg atagatcttt tgaagaactg attaatcgga cgagaataaa
501   gatagagtcc cattctacat gtcaataccg acaacaatga aatttatagt
551   aagaggaaaa tccgtcgact ttaraaatcg kgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details