JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430777.1
Database:	nt	Go to original record
Description:	Haematoxylum brasiletto chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Haematoxylum brasiletto
Original size:	1082 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgaccct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg caaaccaaga agagttcaga
151   aagggagaat aaaaaaggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttgacgacat ttcgtttcgt tagtaaagga atccttccat
251   cgaaactcca gaaaaggaag gatcaaggat gaacatatat acatatacgt
301   actgaaatac tatttcaatt gattagacca gaccccaaat ctcgattttt
351   gaatatttct atgacaaatg aaagatgtga atagattcca agttgaagaa
401   agaatcgaat atttattgat caaatcattc actccatcat agtctgatag
451   atcttttgaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtca ataccgacaa caatgaaatt gatagtaaga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details