JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430776.1
Database:	nt	Go to original record
Description:	Gymnocladus dioica chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Gymnocladus dioicus
Original size:	1066 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	583	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagcgagaat aaaacaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcaaaactcc cgaaaagaaa ggatcaagga tgaacatata tatacgtact
301   gaaatactat ttcaattgat tagacccaaa atctctattt tgtcatattt
351   atatgacaaa tgaaagatgt gaatcgattc caagttgaag aaagaatcga
401   atattcattg atcaaatcat tcactccatc atagtctgat agatcttttg
451   aagaactgat taatcggacg agaataaaga tagagtccca ttctacatgt
501   caataccgac aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details