Match details

Record ID: AF430775.1
Database: nt Go to original record
Description: Gleditsia triacanthos chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Gleditsia triacanthos
Original size: 1090 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 589 + 589 bp

Amplicon: 589 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagcgagaat aaaacaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tctaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccccaaatct ctattttgtc
351   atatttatat gacaaatgaa agatgtgaat cgattccaag ttgaagaaag
401   aatcgaatat tcattgatca aatcattcac tccatcatag tctgatagat
451   cttttgaaga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gattttagaa atcgtgaggg ttcaagtccc tctatcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.65e-08
primerB 100.0 20 bp 0 0 589 570 2.65e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.