Match details

Record ID: AF430774.1
Database: nt Go to original record
Description: Diptychandra aurantiaca chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Diptychandra aurantiaca
Original size: 1063 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 590 + 590 bp

Amplicon: 590 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aaggtagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgatgaca tttcgtttcg ctagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatacg tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccccaaa tccttttttt
351   aatatttata tgaccaatga aagatgtgaa tcgattccaa gttgaagaaa
401   gaatagaata ttcattgatc aaatcattca ctccatcata gtctgaaaga
451   tcttttgaag aactgattaa tcagacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aatgaaattt ctagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.66e-08
primerB 100.0 20 bp 0 0 590 571 2.66e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.