JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430773.1
Database:	nt	Go to original record
Description:	Delonix decaryi chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Delonix decaryi
Original size:	1063 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgattacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaacccc agaaaagaaa ggatcaagga tgaacatacg tatagtactg
301   aaatactatt tcaattgatt agaccagaca gaccccaaat ctctattttt
351   tcatatttat atgagaaatg aaagatgtga atcgattcca agttgaagaa
401   agaatcgaat attcattgat caaatcattc actccatcat agtctgatag
451   atcttttcaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtta ataccggcaa caatgaaatt tatagtaaga ggaaaatccg
551   gcgactttag aaatgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details