JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430771.1
Database:	nt	Go to original record
Description:	Cordeauxia edulis chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Cordeauxia edulis
Original size:	1090 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg gagaataaaa aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttc gtttcgttag taaaggaatc
251   cttccatcga aactccagaa aagaaaggat caaggatgaa catatatacg
301   tatacgtact gaaatactat ttcaattgat tagaccagac agaccccaaa
351   tctctatttt tttaatattt atatgacaaa tgaaagatgt gaatagattc
401   caagttgaag aaagaatcga atatttattg atcaaatcat tcactccatc
451   atagtctgat agatcttttg aagaactgat taatcggacg agaataaaga
501   tagagtccca ttctacatgt caataccgac aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details