Match details

Record ID: AF430768.1
Database: nt Go to original record
Description: Cercis canadensis chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Cercis canadensis
Original size: 1068 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 593 + 593 bp

Amplicon: 593 bp
1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaga
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctac
201   caaatggagt tgacggcatt tcgtttcgtt agtaaagtaa tccttccatc
251   aaaactccat aaaagaaagg atcaaagata aacgtatata tacgtatacc
301   taccgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   tttaatattt atatgagaaa tgaaagatgt aaatagattc aaagttgagg
401   aaaaagtcga atattcattc atcaaatcat tcactccatc atagtccgat
451   agatcttttg aagaactgat taatcggaca cgaataaaga tagagtccca
501   ttctacatgt caataccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt agaaatcgtg agggttcaag tccctctatc ccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.67e-08
primerB 100.0 20 bp 0 0 593 574 2.67e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.