JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430766.1
Database:	nt	Go to original record
Description:	Cenostigma macrophyllum chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Cenostigma macrophyllum
Original size:	1065 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gvttgmgcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcc ttagtaaagg aatccttcca
251   tcgaaacccc agaaaagaaa ggatcaagga tgaacatacg tathgtactg
301   aaatactatt tcaattgatt agaccagaca gaccccaaat ctctattttt
351   tcatatttat atgavaaatg aaagatgtga atcgattcca agttgaagaa
401   agaatcgaat attcattgat caaatcattc actccatcat agtctgatag
451   atcttttcaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtya ataccggcaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgcctttaa aaatcgggag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details