JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430764.1
Database:	nt	Go to original record
Description:	Burkea africana chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Burkea africana
Original size:	1068 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaaagga gttgacgaca ttttgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatacg tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccccaaa tctctatttt
351   ttaatattta tatgacaaat gaaagatgtg aatcgattcc aagttgaaga
401   aagaatcgaa tcttcattga tcaaatcatt cactccatca gagtctgata
451   gatcttttga agaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca acaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details