JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430762.1
Database:	nt	Go to original record
Description:	Batesia floribunda chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Batesia floribunda
Original size:	1088 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aaggaagaat aaaaaaaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg aattgacgac atttcctttc gttagtaaag gaatccttcc
251   atcgaaactc cagaaaagaa aggatcaagg atgaacatat atatacgtac
301   tgaaatacta tttcaattga ttagaccaga cagaccccaa atctctattt
351   tttaatattt atatgaaaaa tgaaagatgt gaatcgattc caagttgaag
401   aaagaatcga atattcattg atcaaatcat tcactccatc atagtctgat
451   agatcttttg aagaactgat taatcggacg agaataaaga tagagtccca
501   ttctacatgt caatatcgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details