JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430761.1
Database:	nt	Go to original record
Description:	Balsamocarpon brevifolium chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Balsamocarpon brevifolium
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	588	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttawttg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacagaaa gggaaaggga
151   gaaaaggata ggtgcagaga ctcaacggaa gctgttctaa caaatggagt
201   tgacgacatt tcgtttcgtt agtaaaggaa tccttccatc gaaactcccg
251   aaaagaaagt atcaaggatg aacatatata tacgtactga aatactattt
301   caattgatta gaccagacag accccaaatc cctatttttg aatatttata
351   tgacaaatga aagatgtgaa tagattccaa gttgaagaaa gaaagaatcg
401   aatatttatt gatcaaatca ttcactccat catagtctga tagatctttt
451   gaagaactga ttaatcggac gagaataaag atagagtccc attctacatg
501   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgactt
551   tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details