Match details

Record ID: AF430758.1
Database: nt Go to original record
Description: Pomaria brachycarpa chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Pomaria brachycarpa
Original size: 1066 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 598 + 598 bp

Amplicon: 598 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac cccaaatctc
351   tattttttaa tatttatatg acaaatgaaa gatgtgaata gattccaagt
401   tgaagaaaga atcgaatatt tattgatcaa atcattcatt ccataatagt
451   ctgatagatc ttttgaagaa ctgattaatc ggacgagaat aaagatagag
501   tcccattcca catgtcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.69e-08
primerB 100.0 20 bp 0 0 598 579 2.69e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.