Match details

Record ID: AF430757.1
Database: nt Go to original record
Description: Pomaria austrotexana chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Pomaria austrotexana
Original size: 1053 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 599 + 599 bp

Amplicon: 599 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac cccaaatctc
351   tattttttta atatttatat gacaaatgaa agatgtgaat agattccaag
401   ttgaagaaag aatcgaatat ttattgatca aatcattcat tccataatag
451   tctgatagat cttttgaaga actgattaat cggacgagaa taaagataga
501   gtcccattct acatgtcaat aacgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.70e-08
primerB 100.0 20 bp 0 0 599 580 2.70e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.