JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430751.1
Database:	nt	Go to original record
Description:	Hoffmannseggia oxycarpa chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Hoffmannseggia oxycarpa
Original size:	936 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgtttttcg aaaaccaaga agagttcaga
151   aagggaaagg gggaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggattaagga tgaacatata tatgtactga
301   aatactattt caattgatta caccagacgg accccaaatc cctttttttt
351   gatatttata tgacaaatga aagatgtgaa tagattccaa gttgaagaaa
401   gaatcgaata tttattgatc aaatcattca ctccatcata gtctgataga
451   tcttttgaag aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details