JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430750.1
Database:	nt	Go to original record
Description:	Hoffmannseggia miranda chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Hoffmannseggia arequipensis
Original size:	1040 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgaccct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc caaatcctgt ttttcgaaaa ccaagaagag
151   ttcagaaagg gaaagggaga aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttc ctttcgttag taaaggaatc
251   cttccatcga aactccagaa aagaaaggat taaggatgaa catatatacg
301   tactgaaata ctatttcaat tgattacacc agacagaccc caaatcccta
351   ttttttaata tttatatgac aaatgaaaga tgtgaataga ttccaagttg
401   aagaaagaat cgaatattta ttgatcaaat cattcactcc atcatagtct
451   gatagatctt ttgaagaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details