JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430737.1
Database:	nt	Go to original record
Description:	Caesalpinia sessilifolia chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Denisophytum sessilifolium
Original size:	1075 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	602	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctatcaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttctg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca ttcctttcgt tagtaaagga atccttccat
251   cgaaactcca gaaaagaaag gatcaaggat gaacatatat atatgtatac
301   gtactgaaat actatttcaa ttgattagac cagacaaacc ccaaatctct
351   attgtcataa tatttatatg acaaatgaaa gatgtgaata gattccaagt
401   tgaagaaaga atcgaatatt tattgatcaa atcattcact ccatcatagt
451   ctgatagatc ttttgaagaa ctgattaatc ggacgagaat aaagatagag
501   tcccattcta catgtcaata acgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg acttttgaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details