JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430733.1
Database:	nt	Go to original record
Description:	Caesalpinia pulcherrima chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Caesalpinia pulcherrima
Original size:	1065 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	599	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca ttttgtttcg ttagtaaagg aatccttcca
251   tggaaactcc agaaaagaaa gtatcaaggg tgaacatata tatacgtact
301   gaaatactat ttcaattgat taagaccaga cagaccccaa atctctattt
351   tttaatattt atatgacaaa tgaaagatgt caatagattc caagttgaag
401   aaagaatcta atatttatat ttattgatca aatcattcaa tccatcatag
451   tctgatagat cttttgaaga actgattaat cggacgagaa taaagataga
501   gtcccattct acatgtcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details