Match details

Record ID: AF430727.1
Database: nt Go to original record
Description: Caesalpinia mexicana chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Erythrostemon mexicanus
Original size: 1057 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 600 + 600 bp

Amplicon: 600 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatatgtata
301   ctgaaatatt atttcaattg attagaccag acagaccccc aatccctatt
351   ttttaatatt tatatgacaa atgaaagatg tgaatagatt ccaagttgaa
401   gaaagaatcg aatatttatt gatcaaatca ttcattccat gataatgata
451   gtctgataaa tcttttgaag aactgattaa tcggacgaga ataaagatag
501   agtcccattc tacatgtcaa taccgacaac aatgaaattt atagtaagag
551   gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.70e-08
primerB 100.0 20 bp 0 0 600 581 2.70e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.