JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430723.1
Database:	nt	Go to original record
Description:	Caesalpinia hauthalii chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Pomaria rubicunda var. hauthalii
Original size:	1071 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac ccccaatctc
351   tattttttaa tatttatatg acaaatgaaa gatgtgaata gattccaagt
401   tgaagaaaga atcgaatatt tattgatcaa atcattcatt ccataatagt
451   ctgatagatc ttttgaagaa ctgattaatc ggacgagaat aaagatagag
501   tcccattcta catgtcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details