Match details

Record ID: AF430716.1
Database: nt Go to original record
Description: Caesalpinia exilifolia chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Erythrostemon exilifolius
Original size: 1072 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 592 + 592 bp

Amplicon: 592 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatcttttca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatgtatact
301   gaaatattat ttcaattgat tagaccagac agactccaaa tctctatttt
351   ttcatattta tatgacaaat gaaagatgtg aatagattcc aagttgaaga
401   aagaatcgaa tatttattga aaaactcatt catttcatga tagtctgata
451   aatcttttga agaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca acaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.67e-08
primerB 100.0 20 bp 0 0 592 573 2.67e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.