Match details

Record ID: AF430714.1
Database: nt Go to original record
Description: Caesalpinia decapetala chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Biancaea decapetala
Original size: 1089 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 615 + 615 bp

Amplicon: 615 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    atataccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   ttgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac cccaaatctc
351   tattttttaa tatttatata acaaaaatat ttatataaca aatgaaagat
401   gtgaatagat tccaagttga agaaagaatc gaatatttat tgatcaaatc
451   attcactcca tcatagtctg atagatcttt tgaagaactg attaatcgga
501   cgagaataaa gatagagtcc cattctacat gtcaataccg acaacaatga
551   aatttatagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
601   agtccctcta tcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.77e-08
primerB 100.0 20 bp 0 0 615 596 2.77e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.