Match details

Record ID: AF430712.1
Database: nt Go to original record
Description: Caesalpinia caudata chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Erythrostemon caudatus
Original size: 1045 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 596 + 596 bp

Amplicon: 596 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagagaact ttcaaattca gagaaaccct ggaattaaca
101   atgggcaatc ctgagccaaa tcctgttttc cgaaaaccaa taagagttca
151   gaaagggaga ataaaaaaag gataggtgca gagactcaac ggaagctgtt
201   ctaacaaatg gagttgacga catttccttt cgttagtaaa ggaatccttt
251   catcgaaact ccagaaaaga aaggatcaag gatgaacata tatatatgta
301   tactgaaata ttatttcagt tgattagacc agacagaccc caaatctcta
351   ttttttcata tttatatgac aaatgaaaga tgtgaataga ttccaagttg
401   aagaaagaat cgaatattta ttgatcaact cattcattcc atgatagtct
451   gataaatatt ttgaagaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc atgagggttc aagtccctct atcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.68e-08
primerB 100.0 20 bp 0 0 596 577 2.68e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.